Outline the setup of a triple quadrupole instrument for the identification and quantification of a drug of known m/z: Advanced Analytical Science Assignment, SHU, UK

University	Sheffield Hallam University (SHU)
Subject	Advanced Analytical Science

Outline the setup of a triple quadrupole instrument for the identification and quantification of a drug of known m/z from liver tissue homogenate that loses a water molecule when subjected to MS/MS analysis.

Explain the benefits of using derivatization in compound-specific IRMS and some of the issues encountered with this approach.

Biopharmaceuticals such as monoclonal antibodies and drug conjugates require careful quality control. Use your knowledge of bioanalytical chemistry to state with a short explanation an approach that could be used to determine the accurate mass of a biopharmaceutical.

Why is high mass resolution important in label-free quantitative proteomics carried out using “bottom-up” techniques?

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